LUNG CANCER
Vysis BRAF SpectrumGold FISH Probe Kit
For more information, contact Abbott.
For more information, contact Abbott.
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The Vysis BRAF SpectrumGold FISH Probe Kit is designed to detect copy number of 7q34 via fluorescence in situ hybridization (FISH) in formalin fixed paraffin embedded (FFPE) lung cancer tissue.
The Vysis BRAF FISH assay is based on the ability of BRAF locus specific identifier (LSI) probe to identify copy number changes of 7q34 chromosomal locus, using a FISH test.
BRAF is one of three serine/threonine RAF-regulated kinases that have an important role in cellular proliferation, differentiation, and programmed cell death. It alsoparticipates in the RAS-RAF-MEK-ERK-BRAF in promoting tumorigenesis (malignant transformation of kinase BRAFs).
Mutationally activated BRAF-V600E is detected in melanoma (70%), colorectal (15%), papillary thyroid (40%), ovarian (30%), and non-small-cell lung cancers (NSCLCs) (3%). Melanoma with activating mutation of BRAF is more likely tohave copy gains at the BRAF locus. BRAF copy number gains have been identified in both follicular thyroid cancer and malignant melanoma, and may occur through either gene amplification or chromosome 7 polysomy. The BRAF copy number gains are expected in lung cancer, where chromosome 7 is also amplified.
The approximately 510 kb (chr7:139912659-140422776; March 2006 assembly, UCSC Genome Browser) LSI BRAF (7q34) SpectrumGold probe contains the entire BRAF gene on chromosome 7.
Normal diploid nuclei or metaphase chromosome sets are expected to exhibit two gold fluorescent BRAF signals, which correspond to two target loci on chromosome homologues to which the BRAF fluorescent probe is bound: 7q34. A chromosome set that has an extra copy (copies) of BRAF (7q34) will exhibit more than two gold fluorescent signals.
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Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.