Vysis EGFR / CEP 7 FISH Probe Kit
For more information, contact Abbott.
For more information, contact Abbott.
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The Vysis EGFR/CEP 7 FISH Probe Kit is designed to detect copy number of the EGFR gene and of centromere 7 via fluorescence in situ hybridization (FISH) in formalin‑fixed, paraffin-embedded tissue specimens. This procedure has been optimized for use with formalin-fixed, paraffin‑embedded (FFPE) lung tissue, but may be applicable for other tissue types. Other tissue types may require adjusted pretreatment, hybridization, and/or wash conditions.
The LSI EGFR Probe is labeled with SpectrumOrange and covers an approximately 303 kb region that contains the entire EGFR gene. The CEP 7 probe, labeled with SpectrumGreen, hybridizes to the alpha satellite DNA located at the centromere of chromosome 7 (7p11.1-q11.1). This probe set is premixed in Hybridization Buffer.
In a cell with normal copy number of the EGFR gene and chromosome 7, two orange signals (EGFR), and two green signals (chromosome 7) will be observed. Simultaneously, the copy number of chromosome 7 can be quantified by enumeration of the green signals observed within the same cell. Therefore, enumeration of both the orange EGFR and green CEP 7 signals provide a mechanism for determining EGFR copy number relative to total chromosome 7 copy number.
Abnormal Hybridization: An abnormal cell hybridized with the Vysis LSI EGFR SpectrumOrange /CEP 7 SpectrumGreen Probes. The cell contains multiple EGFR (orange) signals and chromosome 7 (green signals).
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Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.