CHRONIC LYMPHOCYTIC LEUKEMIA (CLL)
Vysis LSI p53 / LSI ATM and LSI D13S319 / LSI 13q34 / CEP 12 Multi-color Probe Set
For more information, contact Abbott.
For more information, contact Abbott.
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These fluorescence in situ hybridization (FISH) probes are intended to detect deletions of the LSI p53, LSI ATM, and LSI D13S319 probe target sequences. They are also intended for the detection of gain in copy number of the D12Z3 sequence as occurs in trisomy 12.
Probe Set 1 contains a SpectrumOrange LSI p53 (TP53: tumor protein p53 gene) probe and a SpectrumGreen ATM (ataxia telangiectasia mutated gene) probe. The p53 probe located at 17p13.1 contains the complete p53 gene and is about 172 kb in length. The approximately 732 kb ATM probe contains the complete ATM gene which is located at 11q22.3. Probe Set 2 is composed of an approximately 135 kb SpectrumOrange D13S319 (13q14) probe, an approximately 612 kb SpectrumAqua LSI13q34 (13q34) probe, and a SpectrumGreen CEP 12 probe which contains the D12Z3 alpha satellite sequence located at the centromere of chromosome 12.
This multi-color probe set is provided in a two-mixture format.
This probe allows status assessment of the following three chromosome regions: 13q14.3 (D13S319), 13q34, and 12p11.1-q11. In a normal cell with two intact copies of chromosome 13 and chromosome 12, a two orange, two aqua, and two green signal pattern will be observed. In an abnormal cell with chromosome 13 aberrations only, more complex signal patterns may be expected depending upon the nature of the aberration. Monosomy 13 or 13q- will both appear as a one orange, one aqua, two green signal pattern. An interstitial deletion of the 13q14.3 region will appear as either a one orange, two aqua, two green signal pattern (hemizygous deletion) or a two aqua, two green signal pattern (homozygous deletion) (data not shown). In an abnormal cell with chromosome 12 copy number changes, one will observe greater or less than two green signals.
This multi-color probe set is provided in a two-mixture format.
Abnormal Hybridization: An abnormal cell hybridized with Probe Set 2. Both copies of chromosome thirteen and its q arm are intact as indicated by the two orange (LSI D13S319) and two aqua (LSI 13q34) signals. One extra copy of chromosome 12 (trisomy 12) is present as indicated by the three green signals.
Abnormal Hybridization: An abnormal cell hybridized with Probe Set 2. One copy of chromosome 13 is deleted for the D13S319 region as indicated by the single orange signal (LSI D13S319) and the two aqua signals (LSI 13q34). One extra copy of chromosome 12 (trisomy 12) is present as indicated by the three green signals.
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Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.